LEGUME INDIAN BEAN (PHASEOLUS VULGARIS) PARTIALLY PURIFIED ANTI-NUTRITIONAL PROTEIN FACTOR EXHIBIT ANTI-OXIDANT, IMMUNOMODULATORY AND ANTI-CANCER PROPERTIES

Objective: This study was aimed to access one of the hidden ANF in Indian bean and assessment its antioxidant, immunomodulatory, antiangiogenic, anti-cancer properties. Methods: beans were processed evaluated for presence through HA activity. Bean-ANF partially purified by ammonium sulfate precipitation (ASP) dialysis. Obtained Partially Purified bean-ANF (PPb-ANF) resolved on 12% SDS-PAGE, analyzed protease digestibility. The immunogenicity PPb-ANF characterized producing polyclonal antibodies checked natural healthy human sera. Pharmacological studies assessed anti-oxidant, immunogenic, anti-angiogenic assays. Results: showed significant specific activity up 150 Units/mg compared crude extract 9.3 Units/mg. identified have more than 90% homogeneity purity with an observed M W 30-32 kDa. pepsin digestive stability 2 h at 37 ᵒC appears resistant. Rabbit produced anti-PPb-ANF IgG antibody immunization exhibited immunogenic Natural IgE found non-atopic sera against PPb-ANF, indicative immunogenicity. confirmed potent antioxidant At 400 µg highest around DPPH 89% inhibitory lipid peroxidation 70% (3 μg) 40% (1.5 blood vessel density control a (*p<0.05.) reduction solid tumor weight upon dose-2 treatment (50 mg/kg body weight) also increased lifespan 70 d model. Conclusion: as digestive-resistant, hemagglutinin molecular protein is has potential antiangiogenic Further purification mechanistic-based pharmacological characterization might explore this molecule.